Environmental
Analytical challenges are diverse and challenging with wide ranging concentrations of contaminant and matrix properties. UHPLC+ provides a wider range of solutions for these challenges based on x2 Dual LC technology, intelligent instrument control, and integrated on-line SPE-LC solutions. These can all be enhanced with powerful detection techniques such as UV, fluorescence, electrochemistry, mass spectrometry, and charged aerosol using Dionex Corona®. No matter which organic pollutants are of concern, the UHPLC + focused UltiMate® 3000 platform will give the solutions.
A good example of the performance of the UltiMate 3000 Standard is the analysis of Polyaromatic Hydrocarbons (PAHs). Detection is performed with a fast-wavelength switching fluorescence detector, and 17 different PAHs are separated in less than 6 minutes.

Separation of 17 PAHs in Less Than 6 Minutes
Method Conditions
System Used: UltiMate 3000 Standard
Flow: 2 mL/min
Injection Volume: 12.5 µL
Temperature: 30°C
Pressure: 510 Bar
Column Used: Machery-Nagel Nucleodur® C18 PAH
Particle Size: 3 µm
Length: 100 mm
Diameter: 3 mm
Gradient Profile:
| Time [min] | %B |
| 0.000 | 45 |
| 0.500 | 45 |
| 3.762 | 90 |
| 4.275 | 95 |
| 5.288 | 95 |
| 5.523 | 45 |
| 6.200 | 45 |
FLD detection parameters:
| Time [min] | WVLex [nm] | WVLem [nm] | Filter [nm] |
| 0.000 | 255 | 315 | 280 |
| 2.560 | 244 | 360 | 280 |
| 2.750 | 244 | 400 | 370 |
| 2.970 | 237 | 460 | 435 |
| 3.150 | 237 | 385 | 370 |
| 3.400 | 277 | 376 | 280 |
| 4.050 | 255 | 420 | 370 |
| 4.800 | 300 | 415 | 370 |
| 5.264 | 250 | 495 | 435 |
| 5.650 | 225 | 315 | 280 |
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