Environmental

Analytical challenges are diverse  and challenging with wide ranging concentrations of contaminant and matrix properties. UHPLC+ provides a wider range of solutions for these challenges based on x2 Dual LC technology, intelligent instrument control, and integrated on-line SPE-LC solutions. These can all be  enhanced with powerful detection techniques such as UV, fluorescence, electrochemistry, mass spectrometry, and charged aerosol using Dionex Corona®. No matter which organic pollutants  are of concern, the UHPLC + focused UltiMate® 3000 platform will give the solutions.

A good example of the performance of the UltiMate 3000 Standard is the analysis of Polyaromatic Hydrocarbons (PAHs). Detection is performed with a fast-wavelength switching fluorescence detector, and 17 different PAHs are separated in less than 6 minutes.

Separation of 17 PAHs in Less Than 6 Minutes

Method Conditions

System Used: UltiMate 3000 Standard

Flow: 2 mL/min
Injection Volume: 12.5 µL
Temperature: 30°C
Pressure: 510 Bar

Column Used: Machery-Nagel Nucleodur® C18 PAH
Particle Size: 3 µm
Length: 100 mm
Diameter: 3 mm

Gradient Profile:

Time [min]%B
0.00045
0.50045
3.76290
4.27595
5.28895
5.52345
6.20045

FLD detection parameters:

Time [min]WVLex [nm]WVLem [nm]Filter [nm]
0.000255315280
2.560244360280
2.750244400370
2.970237460435
3.150237385370
3.400277376280
4.050255420370
4.800300415370
5.264250495435
5.650225315280


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Chromeleon 7.1 SR 1 provides new home ePanels built dynamically according to the configuration of the system. This video shows the new home ePanel and its new features for the UltiMate 3000.